Potential Improvement of Hydraulic Resistance in Coastal Soils Using Optimized Microbially Induced Calcium Carbonate Precipitation (MICP): A Case Study of Kish Island
Keywords:
MICP, Sporosarcina bacteria, calcium carbonate, optimization, central composite designAbstract
In this study, the efficiency of the microbially induced calcium carbonate precipitation (MICP) process using the bacterium Sporosarcina pasteurii was evaluated for improving the hydro-geochemical properties of the soil of Kish Island with the objective of reducing saline water intrusion from the Persian Gulf. Accordingly, the effects of three independent variables, namely urea concentration, calcium chloride concentration, and reaction time, were optimized with respect to response variables including effluent volume, electrical conductivity, and pH. The optimization results indicated that the minimum effluent volume of 5 mL was obtained at a urea concentration of 0.5 M, a calcium chloride concentration of 0.5 M, and a reaction time of 9 days. In addition, the minimum electrical conductivity value of 69.5 dS/m occurred at a urea concentration of 0.5 M, a calcium chloride concentration of 0.5 M, and a reaction time of 15 days. Finally, the maximum pH value of 7.65 was recorded at a urea concentration of 0.5 M, a calcium chloride concentration of 0.5 M, and a reaction time of 15 days. Based on the findings of this research, the application of Sporosarcina pasteurii for the formation of a biological barrier can be introduced as a promising approach for preventing the advancement of saline water from the Persian Gulf into the soil of Kish Island.
References
Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2011). Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. Journal of Industrial Microbiology & Biotechnology, 36(7), 981-988. https://doi.org/10.1007/s10295-009-0578-z
Al-Thawadi, S. (2008). High strength in-situ biocementation of soil by calcite precipitating locally isolated ureolytic bacteria Murdoch University]. https://researchportal.murdoch.edu.au/esploro/outputs/doctoral/High-strength-in-situ-biocementation-of-soil/991005544552507891
Chen, X., & Achal, V. (2020). Effect of simulated acid rain on the stability of calcium carbonate immobilized by microbial carbonate precipitation. Journal of Environmental Management, 264. https://doi.org/10.1016/j.jenvman.2020.110419
Choi, S. G., Chu, J., Brown, R. C., Wang, K. J., & Wen, Z. Y. (2017). Sustainable biocement production via microbially induced calcium carbonate precipitation: Use of limestone and acetic acid derived from pyrolysis of lignocellulosic biomass. Acs Sustainable Chemistry & Engineering, 6(5). https://doi.org/10.1021/acssuschemeng.7b02137
Clarà Saracho, A., Haigh, S. K., Hata, T., & et al. (2020). Characterisation of CaCO3 phases during strain-specific ureolytic precipitation. Scientific reports. https://doi.org/10.1038/s41598-020-66831-y
DeJong, J. T., Mortensen, B. M., Martinez, B. C., & Nelson, D. C. (2010). Bio-mediated soil improvement. Ecological Engineering, 36(2), 197-210. https://doi.org/10.1016/j.ecoleng.2008.12.029
Dong, J., Xue, J., Wang, W., Ma, J., & Wang, Z. (2025). Assessment of seawater intrusion in coastal aquifers by modified CCME-WQI Indicators: Decadal dynamics in North Jiaozhou Bay, China. Ecological Indicators. https://doi.org/10.1016/j.ecolind.2025.113591
Fujita, M., Nakashima, K., Achal, V., & Kawasaki, S. (2017). Whole-cell evaluation of urease activity of Pararhodobacter sp. isolated from peripheral beachrock. Biochemical Engineering Journal. https://doi.org/10.1016/j.bej.2017.04.004
Gat, D., Ronen, Z., & Tsesarsky, M. (2017). Long-term sustainability of microbial-induced CaCO3 precipitation in aqueous media. Chemosphere, 184, 524-531. https://doi.org/10.1016/j.chemosphere.2017.06.015
Guo, L., Wang, B., Guo, J., Guo, H., Jiang, Y., Zhang, M., & Dai, Q. (2024). Experimental study on improving hydraulic characteristics of sand via microbially induced calcium carbonate precipitation. Geomechanics and Energy Environment. https://doi.org/10.1016/j.gete.2023.100519
Haihe, Y., Zheng, T., Jia, Z., Su, T., & Wang, C. (2021). Study on the influencing factors and mechanism of calcium carbonate precipitation induced by urease bacteria. Journal of Crystal Growth. https://doi.org/10.1016/j.jcrysgro.2021.126113
Hammes, F. (2003). Ureolytic microbial calcium carbonate precipitation Ghent University].
Harkes, M. P., van Paassen, L. A., Booster, J. L., Whiffi, V. S., & van Loosdrecht, M. C. M. (2010). Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecological Engineering, 36(2), 112-117. https://doi.org/10.1016/j.ecoleng.2009.01.004
Khoshtinat, S., Marano, C., & Kioumarsi, M. (2024). Computational Model of the Effect of pH on Calcium Carbonate Precipitation by Sporosarcina pasteurii. Discover Materials. https://doi.org/10.1007/s43939-025-00241-7
Konstantinou, C., & Wang, Y. (2023). Unlocking the Potential of Microbially Induced Calcium Carbonate Precipitation (MICP) for Hydrological Applications: A Review of Opportunities, Challenges, and Environmental Considerations. Hydrology. https://doi.org/10.3390/hydrology10090178
Konstantinou, C. H., & Wang, Y. (2024). Statistical and machine learning analysis for the application of microbially induced carbonate precipitation as a physical barrier to control seawater intrusion. Journal of Contaminant Hydrology, 263, 104337. https://doi.org/10.1016/j.jconhyd.2024.104337
Lai, H. J., Cui, M. L., & Chu, J. (2022). Effect of pH on soil improvement using one-phase- low-pH MICP or EICP biocementation method. Acta Geotechnica. https://doi.org/10.1007/s11440-022-01759-3
Maleki Kaklar, M., & Yavari, M. (2019). Field applications of microbial precipitation of calcium carbonate in soil improvement; challenges and opportunities. Journal of Water and Soil Sciences. https://jstnar.iut.ac.ir/article-1-3825-fa.html
Meyer, F. D., Bang, S., Min, S., Stetler, L. D., & Bang, S. S. (2011). Microbiologically-induced soil stabilization: Application of Sporosarcina Pasteurii for fugitive dust control. Geo-Frontiers Congress: Advances in Geotechnical Engineering,
Mori, D., & Uday, K. V. (2021). A review on qualitative interaction among the parameters affecting ureolytic microbial-induced calcite precipitation. Environmental Earth Sciences. https://doi.org/10.1007/s12665-021-09613-7
Mostafa, S., Khajeh, S. A., & Aydin, B. (2016). Bioconcrete: Next generation of self-healing concrete. Applied Microbiology and Biotechnology, 100(6). https://doi.org/10.1007/s00253-016-7316-z
Nafeesa, S., Amna, J., Fazal, A., & Rao, A. K. (2021). Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO3 precipitation in bio-based cementitious composites. Microbial Biotechnology. https://pubmed.ncbi.nlm.nih.gov/33629805/
Nasiri, M., Hamidi, M., & Kardan Moghadam, H. (2019). Simulation of saltwater intrusion in coastal aquifers (Case study: Southern coasts of the Caspian Sea). Water and Soil, 34(2), 269-286. https://www.researchgate.net/publication/343548918_Simulation_of_Seawater_Intrusion_in_Coastal_Aquifers_Case_Study_the_Southern_Shores_of_the_Caspian_Sea
Ng, W. S., Lee, M. L., & Hii, S. L. (2012). An overview of the factors affecting microbial-induced calcite precipitation and its potential application in soil improvement. World Academy of Science, Engineering and Technology, 62, 723-729. https://www.semanticscholar.org/paper/An-Overview-of-the-Factors-Affecting-Calcite-and-in-Ng-Lee/204b6a9bfc98a91536fdf7e38b104d17b067e232
Okwadha, G. D., & Li, J. (2010). Optimum conditions for microbial carbonate precipitation. Chemosphere, 81(9), 1143-1148. https://doi.org/10.1016/j.chemosphere.2010.09.066
Qabany, A. A., Soga, K., & Santamarina, J. C. (2012). Factors Affecting Efficiency of Microbially Induced Calcite Precipitation. Journal of Geotechnical and Geoenvironmental Engineering. https://ascelibrary.org/doi/10.1061/%28ASCE%29GT.1943-5606.0000666
Seifan, M., & Berenjian, A. (2019). Microbially induced calcium carbonate precipitation: a widespread phenomenon in the biological world. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-019-09861-5
Shahabinejad Raberi, M., Moazameh, N., Najafi, P., Radnejad, H., & Ahmadi Nadushan, M. (2015). Predicting the Salinity of Groundwater in Kish Island Using Artificial Neural Network Methods. National Conference on Environmental Engineering and Management,
Soyson, A., Pungrasmi, W., & Likitlersuang, S. (2021). Efficiency of microbially-induced calcite precipitation in natural clays for ground improvement. Construction and Building Materials. https://doi.org/10.1016/j.conbuildmat.2021.122722
Su, Q., Kambale, R. D., Tzeng, J. H., Amy, G. L., Ladner, D. A., & Karthikeyan, R. (2025). The growing trend of saltwater intrusion and its impact on coastal agriculture: Challenges and opportunities. Science of The Total Environment. https://www.sciencedirect.com/science/article/pii/S0048969725003353
Tianzheng, F., Clara Saracho, A., & Haigh, S. K. (2023). Microbially induced carbonate precipitation (MICP) for soil strengthening: A comprehensive review. Biogeotechnics. https://doi.org/10.1016/j.bgtech.2023.100002
Wang, K. D., Wu, S. F., & Chu, J. (2023). Mitigation of soil liquefaction using microbial technology: An overview. Biogeotechnics, 1(1). https://doi.org/10.1016/j.bgtech.2023.100005
Whiffin, V. S., Van Paassen, L. A., & Harkes, M. P. (2007). Microbial carbonate precipitation as a soil improvement technique. Geomicrobiology Journal, 24(5), 417-423. https://doi.org/10.1080/01490450701436505
Zehner, J., Royne, A., Wentzel, A., & Sikorski, P. (2020). Microbial-induced calcium carbonate precipitation: an experimental toolbox for in-situ and real time investigation of micro-scale pH evolution. Royal Society of Chemistry. https://doi.org/10.1101/2020.04.15.042168
Zhang, J. Z., Tang, C. S., Lv, C., Zhou, Q. Y., & Shi, B. (2024). Monitoring and Characterizing the Whole Process of Microbially Induced Calcium Carbonate Precipitation Using Electrical Resistivity Tomography. Journal of Geotechnical and Geoenvironmental Engineering. https://doi.org/10.1061/JGGEFK.GTENG-11782
Zhang, Q., Ye, W. M., Liu, Z. R., Wang, Q., & Chen, Y. G. (2022). Advances in soil cementation by biologically induced calcium carbonate precipitation. Rock and Soil Mechanics, 43(2), 345-357. https://doi.org/10.16285/j.rsm.2022.02.005
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Copyright (c) 2026 Alireza Zeynali (Author); Somayeh Taheri; Hossein Ali Alikhani, Seyed Saeid Hosseini (Author)

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